Animal Model

Divisi Animal Model 

Model hewan coba merupakan salah satu alat pendukung dalam mempelajari penyakit pada manusia, mempelajari mekanisme obat maupun mengembangkan obat baru. Model hewan coba yang yang valid dan prediktif akan mampu menjawab pertanyaan klinis yang ada.

Secara garis besar, pengembangan model hewan untuk mempelajari suatu penyakit dilakukan menggunakan 3 cara yaitu :

  1. Induksi farmakologi
  2. Induksi operasi
  3. Modifikasi genetik

Masing-masing cara tersebut memiliki kelebihan dan kekurangan masing-masing.

Sampai saat ini model yang paling banyak digunakan di FKKMK UGM adalah model hewan yang dibuat melalui induksi farmakologi dan operasi. Model hewan untuk mempelajari kanker kolon, kanker payudara, diabetes, hipertensi dan gagal ginjal merupakan beberapa dari model hewan yang diinduksi secara farmakologi.

Sedangkan beberapa contoh model hewan yang diinduksi secara operasi yang sudah dikembangkan sampai saat ini di FKKMK UGM adalah model hewan gagal ginjal akut maupun kronik, hipertensi, iskemik global dan anastomosis usus. Sayangnya model hewan dari hasil modifikasi genetik belum digunakan di FKKMK karena fasilitas ruang hewan coba yang belum memungkinkan untuk memfasilitasi genetic modified animal.

Namun penelitian dan usaha untuk mengembangkan genetic modified animal sudah mulai dilakukan dengan cara melakukan penelitian in vitro untuk membuat sel knock out maupun transgenic oleh civitas akademika di FKKMK.

(dr. Dwi Aris Agung, M.Sc, PhD)

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                                    PUBLIKASI
  1. Sari DCR, Aswin S, Susilowati R, etal. (2014). Ethanol Extracts of Centella asiatica Leaf Improves Memory Performance in Rats after Chronic Stress via Reducing Nitric Oxide and Increasing Brain-Derived Neurotrophic Factor (BDNF) Concentration, GSTF. International Journal of Psychology. 1(1):9.
  2. Arfian N, Sari DCR, Romi MM, Wibisono DP, Emoto N. (2015). Heparanase expression in renal interstitial may contribute to epithelial and endothelial cells injuries after kidney ischemic/reperfusion episode in mice. KnE Life Science. 2:70-77
  3. Budiharjo S, Arfian N, Wijaya R, Sari DCR, Prakosa D, Romi MM. (2015). Strain gauge sensors implemented in a new female torso model increases student motivation and confident in gynecologic bimanual examination on clinical skill training. Applied Mechanics and Materials. 771:149-154
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  5. Arfian N, Muflikhah K, Soeroyo SK, Sari DCR, Tranggono U, Anggorowati N. Romi MM. Vitamin D Attenuates Kidney Fibrosis via Reducing Fibroblast Expansion, Inflammation, and Epithelial Cell Apoptosis. Kobe Journal of Medical Sciences (2016). 62(2):38-44. PMID: 27578035
  6. Cahyawati PN. Ngatidjan, Sari DCR, Romi MM, Arfian N. Simvastatin Attenuates Renal Failure In Mice With A 5/6 Subtotal Nephrectomy; International Journal Of Pharmacy And Pharmaceutical Sciences. (2017). 9(5). 12-17
  7. Setyaningsih WAW, Arfian N, Suryadi E, Romi MM, Trianggono U, Sari DCR. Hyperuricemia Induces Wnt5a/Ror2 Gene Expression, Epithelial–Mesenchymal Transition (EMT), and Kidney Tubular Injury in Mice.(2018). Iran Journal of Medical Science.43(2):164-173. PMID: 29749985
  8. Anggorowati N, Ratna Kurniasari Ch, Damayanti K, Cahyanti T, Widodo I, Ghozali A, Romi MM, Sari DC, Arfian N. Histochemical and Immunohistochemical Study of α-SMA, Collagen, and PCNA in Epithelial Ovarian Neoplasm. (2017). Asian Pacific Journal of Cancer Prev.18(3):667-671, PMID: 28440973
  9. Anggorowati N, Ghozali A, Irianiwati, Sari DCR, Romi MM, Arfian N. Upregulation of Endothelin-1/endothelin A receptor expression correlates with heparanase expression in ovarian carcinoma. (2018). Iranian Journal of medical sciences. 43(3):286-295. PMID: 29892146
  10. Romi MM, Arfian N, Tranggono U, Setyaningsih WAW, Sari DCR. Uric acid induces kidney injury through inducing fibroblast expansion, Endothelin-1 expression, and inflammation. (2017). BMC Nephrology.18(1):326. PMID: 29089036
  11. Wirasto RT, Supriyanto I, Prassetyo A et al. Impulsivity and novel object recognition test of rat model for vascular cognitive impairment after antipsychotics treatment. (2016). Journals of Medical Science. 48(3)
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  13. Harahap U, Hasibuan PAZ, Sitorus P, Arfian N, Satria D. Antimigration Activity of an Ethylacetate Fraction of Zanthoxylum acanthopodium DC. Fruits in 4T1 Breast Cancer Cells. (2018) Asian Pacific Journal of Cancer Prev. 19(2):565-569. PMID: 29481017
  14. Arfian N, Kusuma MHH, Anggorowati N, Nugroho DB, Jeffilano A, Suzuki Y, Ikeda K, Emoto N. Vitamin D upregulates endothelin-1, ETBR, and eNOS mRNA expressions and attenuates vascular remodelling and ischemia in kidney fibrosis model in mice. (2018). Physiological Research.67:S137-S147. PMID: 29947534
  15. Hartopo AB, N. Arfian, K. Nakayama, Y. Suzuki, K. Yagi, N. Emoto. Endothelial-Derived Endothelin-1 Promotes Pulmonary Vascular Remodeling In Bleomycin-Induced Pulmonary Fibrosis. (2018). Physiological Research. 67 (Suppl. 1): S185-S197. PMID: 29947539
  16. Sari DCR, Juananda D, Ar-Rochmah M, Romi MM, Arfian N. Ethanolic extract of the Centella asiatica (L.) Urb. leaf decreases cerebellar brain-derived neurotrophic factor (BDNF) levels in rats after chronic stress. (2018). Journal of the Medical Sciences. 50(2):127-137
  17. Hermawati E, Arfian N, Mustofa, Partadiredja G. Spatial Memory Disturbance Following Transient Brain Ischemia is Associated with Vascular Remodeling in Hippocampus. (2018). Kobe Journal of Medical Sciences. 64(1):99-106
  18. Haryono A, Nugrahaningsih DAA, Sari DCR, Romi MM, Arfian N. Reduction of Serum Uric Acid Associated with Attenuation of Renal Injury, Inflammation and Macrophages M1/M2 Ratio in Hyperuricemic Mice Model. (2018). Kobe Journal of Medical Sciences. 64(1):107-114